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产品详情
  • 产品名称:微电极拉制仪

  • 产品型号:Micropipette Puller P-97
  • 产品价格:0
  • 折扣价格:0
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简单介绍:
美国Sutter公司微电极拉制仪可分为垂直式拉制仪和水平式拉制仪.垂直式微电极拉制仪是利用金属丝(钨丝或铂丝绕成螺管状)通过大电流使玻璃管加热到熔点,利用重力拉断.水平微电极拉制仪则是利用弹力或电动力作单次或多次拉制.这后一种拉制仪可用于拉制胞内电极,通常是采用微处理器控制其拉制过程,也有用激光工艺进行拉制的,因而可以拉制石英玻璃管.
详情介绍:
       玻璃微电极是膜片钳系统通过探头直接与细胞膜封接的部件,微电极主要是用不同的玻璃毛坯管,通过控制微电极拉制仪的温度,拉力,拉制时间等参数拉制而成.一般拉制仪可分为垂直式和水平式.垂直式的拉制仪是利用金属丝(钨丝或铂丝绕成螺管状)通过大电流使玻璃管加热到熔点,利用重力拉断.水平拉制仪则是利用弹力或电动力作单次或多次拉制.这后一种拉制仪可用于拉制胞内电极,通常是采用微处理器控制其拉制过程,也有用激光工艺进行拉制的,因而可以拉制石英玻璃管.
美国Sutter公司的P-97程控水平微电极拉制仪常用于各种玻璃,石英等微电极的拉制制作,作为在实验中常用的玻璃,石英等微电极,在电生理,细胞注射的实验中广泛被应用,而美国Sutter公司的P-97程控水平微电极拉制仪被确定为电生理,细胞注射的实验室的标准配置仪器。

性能指标:

1. 湿度控制室                                                      
2. 程控气压
3. 可存贮多达100个程序
4. 写保护锁和日期封印
5. 每次拉制都产生两个对称的电极
6. 为胞内记录和膜片钳电极设计的例子程序可以修改,可对特殊需求进行编程
7. Ramp检测--当使用新灯丝或新玻璃时能对加热设置进行自检验
8. 为灯丝和拉制螺线管提供恒流电源
9. 为拉制膜片钳用电极设计的循环周期拉制
10. 真空荧光显示
11. 自带的供气装置有湿度控制和过滤系统
12. 能拉制稳定、可靠的尖端小于0.1微米的电极
13. 能控制空气释放的时间和压力
14. 内部存储器检验
15. 优化的速度检测回路有*大的敏感度和重复性
16. 质量控制:电镜检测电极尖端变化小于0.1微米,一般大约为0.06微米

P-97微电极拉制仪
(微电极拉制器)
P-97水平微电极拉制仪,制出的电极光滑齐整,无须研磨。
   
P-97微电极拉制器是*新的Flaming/Brown型微电极、膜片钳电极、微注射针拉制器。秉承了P-87的许多优势,P-97进一步提升了机械、电气和软件的设计。拉制过程更易控制,可重复性水平更高。P-97将成熟的可编程的微处理器整合于可靠的机械系统中。可编程控制的拉制参数使得研究人员在大范围的玻璃成分和尺寸设计特定应用电极。
   
在P-97的设计中综合了许多新的机械特性。*明显的一个是包在加热丝周围的腔体。湿度控制仓设计用于将拉制电极中因为湿度的改变对重复性的影响*小化。功率上较先前版本提升了25%,可用于更大的加热丝,更大直径玻璃管,和多管玻璃。钳住加热丝的金属夹重新设计是热量残留*小化。送风开关为大直径和多管玻璃提供外部冷却。增加弹簧夹紧机构,易于固定玻璃电极。增加真空荧光显示便于从各方向观察。
   
新软件提高包括加热时间的显示,有助于程序扩展和故障诊断。可*多写100个程序,存储在内存中,使P-97对于众多用户具有更大用途。现在程序可以设置写保护;此增加的保护防止程序被更改和不注意的删除。程序写好后编程和编辑日期也存储其中。大气压也是编程中的一项。
   
在固定拉力下玻璃变软和开始分离时拉动的速度是被测量的。初始的拉制速度由于玻璃的粘度作用停止上升,其次是玻璃温度的作用。可调节的速度可使玻璃温度选择**到拉力过大的关键点上。拉制仪按照加热丝快速冷却的原理,使尖细电极具有特别的短锥,这样产出的电极特别坚硬,适于磨成斜角。短锥的另一个好处是连接的双管电极降低尖端电阻减小电容。
   
P-97包含弯曲测试克服新加热丝安装后程序调整的困难,及有助于新玻璃管与加热丝结合特性的确定。此测量有助于在设立程序时调整加热温度值,在使用新尺寸和新成分的玻璃管时为温度设置提供评估。
    
正如所有的SUTTER仪器一样,其认真设计的电气部分*大限度的降低了噪声。
1)   程控改变气压。
2)   气源带过滤系统和湿度控制。
3)   所拉玻璃微电极尖端直径小于0.1μm。
4)   拉制大直径或多管玻璃时,供气可选择冷却模式。

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