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产品详情
  • 产品名称:BLS细胞融合仪

  • 产品型号:CF-150C
  • 产品价格:0
  • 折扣价格:0
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简单介绍:
CF-150C细胞融合仪器专为哺乳动物胚胎分裂球电融合而设计,此细胞融合仪可在电解液或非电解液中运用。此细胞融合仪的主要应用是产生小鼠四倍体胚胎产生和体细胞核转移克隆。
详情介绍:

欧盟生物实验室设备与维护集团(Biologica lLaboratory Equipment, Maintenance and Service Ltd.简称:BLS), BLS公司是欧洲一家专业生产电融合设备的厂家,尤其在胚胎干细胞的电融合上面更具有全球独一 无二的技术。BLS产品的用户遍布全球,用我们的设备发表的文章每年多达数百篇。在CELL,NATURE以及NUCLEARACID等专业杂志上经常可以见到BLS矫健的身影。其提供的大量文献与PROTOCOL给用户的科研工作带来极大的方便。



随着ES细胞遗传控制技术的发展,ES衍生细胞胚胎有了更广阔的应用空间:
1、应用于测试新衍生的或遗传控制的胚胎干细胞的发育潜能(Nagy et al., 1993).
2、完全ES衍生细胞胎儿为不同功能或遗传研究提供丰富的组织和器官前身资源(Forrester et al. 1991).
3、*近,gene-trap战略用于完全衍生ES胚胎聚合,此胚胎由ES四倍体细胞胚胎产生,从而获取基因表达模式的直接信息CF-150C细胞融合仪和专业设计的高精度电极槽一起使用。槽有三种不同的缝间隙,250µm,500µm和1000µm,型号名分别为GSS-250,GSS-500和GSS-1000。在做双细胞期分裂球融合时,任何一种槽均可使用。如果用于克隆目的,我们推荐GSS-250。电极材料是高品质不锈钢的,并将其安置在一种厚玻璃中。所有这些材料具有生物安 全性,无毒。


CF-150C细胞融合仪器和专业设计的电极夹GSH-1一起使用.此“快速夹”设计使得电极牢固夹在组织培养板(或其盖上)。在融合过程中优良不会有偶然的滑动,并且给电极线提供了永 久的良好接触。电极夹可用于三种里任何一种电极GSS-250,GSS-500orGSS-1000。


CF-150C细胞融合仪器和新型设计的聚合针DN-10一起使用.在组织培养板底部形成一个小凹陷以便小胚胎的聚集,例如胚胎、ES细胞块以及移植早期的鼠胚胎。和附件球一起使用,这些针头更符合人体动力学,在使用中减少手指和手的压力。
CF-150C细胞融合仪器和聚合针头DN-09一起使用.聚合针DN-09是*初设计的聚合针头,在组织培养板底部形成一个小凹陷以便小胚胎的聚集,例如胚胎、干细胞以及移植早期的鼠胚胎。
如果所有参数都选择**(完全按照BLS的protocol),融合率将高于90%,胚胎在融合过程中死亡率低于10%,并且24小时内至少80%成功融合的胚胎将成长为
健康的四细胞期胚胎。


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